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STEMCELL Technologies Inc methylcellulose-based medium methocult gf h4434
Methylcellulose Based Medium Methocult Gf H4434, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methocult+gf+h4434/methocult/pm39179669-52-21-25
Average 90 stars, based on 1 article reviews
methylcellulose-based medium methocult gf h4434 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Fast recovery of platelet production in NOD/SCID mice after transplantation with ex vivo expansion of megakaryocyte from cord blood CD34+ cells.
Article Snippet: CFU‐Mk assay: Totally, 1 × 104 CD34+ cells were seeded into 24‐well plates for semisolid culture (MethoCult GF H4434, Stem Cell Technologies, Vancouver, Canada) with different cytokines for 2 weeks.

Article Title: The landscape of RNA-chromatin interaction reveals small non-coding RNAs as essential mediators of leukemia maintenance
Article Snippet: MethoCult H4230 and MethoCult GF H4434 (both from STEMCELL Technologies) were used for leukemia cell lines and primary cells, respectively.

Labeling:

Article Title: Treatment of brain damage using umbilical cord blood cells
Article Snippet: FACS analysis using anti-CD34 and anti-CD133 antibodies (Miltenyi Biotec, Gladbach, Germany) labeled with phycoerythrin (PE) (Becton Dickinson, USA) of MACS-sorted cells showed that 90%±3% of the selected cells were positive for both CD34 and CD133 as shown in FIG. 1B. .. Then, cells labeled with 1 μg/mL bisbenzimide (Hoechst 33342; Sigma, USA), resuspended in 3004 Iscove's Modified Dulbecco's Medium (Gibco/Invitrogen, UK) plus 2% FBS (Hyclone, Road Logan, Utah) were mixed with 3 ml MethoCult GF H4434 containing recombinant cytokines and erythropoietin (StemCell Technologies, USA) at 37° C. in a humidified atmosphere of 5% CO2/95% air and antibiotics for one hour, and prepared for transplantation. .. Rat Experimental Stroke Model. Thirty adult male Sprague-Dawley rats weighing about 250-300 g were subjected to three-vessel ligation.

Modification:

Article Title: Treatment of brain damage using umbilical cord blood cells
Article Snippet: FACS analysis using anti-CD34 and anti-CD133 antibodies (Miltenyi Biotec, Gladbach, Germany) labeled with phycoerythrin (PE) (Becton Dickinson, USA) of MACS-sorted cells showed that 90%±3% of the selected cells were positive for both CD34 and CD133 as shown in FIG. 1B. .. Then, cells labeled with 1 μg/mL bisbenzimide (Hoechst 33342; Sigma, USA), resuspended in 3004 Iscove's Modified Dulbecco's Medium (Gibco/Invitrogen, UK) plus 2% FBS (Hyclone, Road Logan, Utah) were mixed with 3 ml MethoCult GF H4434 containing recombinant cytokines and erythropoietin (StemCell Technologies, USA) at 37° C. in a humidified atmosphere of 5% CO2/95% air and antibiotics for one hour, and prepared for transplantation. .. Rat Experimental Stroke Model. Thirty adult male Sprague-Dawley rats weighing about 250-300 g were subjected to three-vessel ligation.

Recombinant:

Article Title: Treatment of brain damage using umbilical cord blood cells
Article Snippet: FACS analysis using anti-CD34 and anti-CD133 antibodies (Miltenyi Biotec, Gladbach, Germany) labeled with phycoerythrin (PE) (Becton Dickinson, USA) of MACS-sorted cells showed that 90%±3% of the selected cells were positive for both CD34 and CD133 as shown in FIG. 1B. .. Then, cells labeled with 1 μg/mL bisbenzimide (Hoechst 33342; Sigma, USA), resuspended in 3004 Iscove's Modified Dulbecco's Medium (Gibco/Invitrogen, UK) plus 2% FBS (Hyclone, Road Logan, Utah) were mixed with 3 ml MethoCult GF H4434 containing recombinant cytokines and erythropoietin (StemCell Technologies, USA) at 37° C. in a humidified atmosphere of 5% CO2/95% air and antibiotics for one hour, and prepared for transplantation. .. Rat Experimental Stroke Model. Thirty adult male Sprague-Dawley rats weighing about 250-300 g were subjected to three-vessel ligation.

Transplantation Assay:

Article Title: Treatment of brain damage using umbilical cord blood cells
Article Snippet: FACS analysis using anti-CD34 and anti-CD133 antibodies (Miltenyi Biotec, Gladbach, Germany) labeled with phycoerythrin (PE) (Becton Dickinson, USA) of MACS-sorted cells showed that 90%±3% of the selected cells were positive for both CD34 and CD133 as shown in FIG. 1B. .. Then, cells labeled with 1 μg/mL bisbenzimide (Hoechst 33342; Sigma, USA), resuspended in 3004 Iscove's Modified Dulbecco's Medium (Gibco/Invitrogen, UK) plus 2% FBS (Hyclone, Road Logan, Utah) were mixed with 3 ml MethoCult GF H4434 containing recombinant cytokines and erythropoietin (StemCell Technologies, USA) at 37° C. in a humidified atmosphere of 5% CO2/95% air and antibiotics for one hour, and prepared for transplantation. .. Rat Experimental Stroke Model. Thirty adult male Sprague-Dawley rats weighing about 250-300 g were subjected to three-vessel ligation.



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